Thioglycollate-elicited macrophages
WebMacrophages were separated from thioglycollate-elicited peritoneal cells. Lymphocytes were purified from peripheral blood and were then stimulated w … Using tumor necrosis factor (TNF)-sensitive L929 cells, TNF-like activity was assayed in the supernatants of feline macrophage and lymphocyte cultures. WebMay 9, 2002 · a, Thioglycollate-elicited peritoneal macrophages or P388D1 cells were surface-labelled with biotin, immunoprecipitated with the 2422 antibody, and analysed by western blotting with peroxidase ...
Thioglycollate-elicited macrophages
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WebFeb 28, 1997 · Abstract. Thioglycollate medium (TGM) is widely used as a stimulatory agent to induce non-infectious peritoneal inflammation for elicitation of macrophages from … WebApr 9, 2024 · Thioglycolate-elicited macrophages were obtained from the peritoneal cavity of male C57BL6/6J mice as previously described . Briefly, mice were intraperitoneally injected with 3 mL of 3% thioglycolate (BD Biosciences Japan, Tokyo, Japan). Four days later, the exudate cells in the peritoneal cavity were collected by washing with PBS.
WebResident peritoneal macrophages can readily be harvested from mice and purified by adherence to tissue culture plastic. The injection of Bio-Gel polyacrylamide beads or thioglycollate broth into the peritoneal cavity produces an inflammatory response allowing the purification of large numbers of elicited macrophages. Webthioglycollate-elicited macrophages expressed Ki67 and only 0.2% were in S phase (Fig. 2A). Notably, pleural macrophages isolated 10 days after infection with L. sigmodontis exhibited a substantial increase in the frequency of dividing cells. On average, 38% of cells in the pleural cavity were positive for Ki67 and 6% were in S phase (Fig. 2B).
WebSep 20, 2015 · Here we described a method for the isolation of peritoneal macrophages from thioglycollate-elicited mice and alternative activation by stimulation with IL-4. Discover the world's research. WebNov 11, 2024 · Thioglycollate-elicited macrophages were allowed to attach on a coverglass for 30 min and then fixed by 4% PFA in PBS for 1 h. Cells were stained for 30 min with 0.3% ORO solution in 60% isopropanol. The mean area and integrated optical density of ORO-stained region per cell were quantified with 200 representative cells using ImageJ …
WebJul 21, 2014 · Thioglycollate-elicited peritoneal and bone marrow-derived macrophages are commonly used ex vivo systems to study primary macrophage function. In this study, we reveal dramatic baseline differences in the lysosome morphology and function between these macrophage populations and provide evidence that these differences can be …
WebI have been trying to isolate protein from thioglycolate-elicited peritoneal macrophages. After harvest, I plate cells at ~1-2mil/well, wash out non-macrophages the next day, and wait till ... god of war cliffs of the raven winds of helWebIn contrast to thioglycolate-elicited macrophages, naïve peritoneal and bone marrow-derived atg7 −/-macrophages exhibited no LC3-II, even under inflammatory stimuli in vitro. Hence, … booker t washington alumni associationWebJun 10, 2015 · Cholesterol assays in macrophages. Thioglycollate-elicited peritoneal macrophages (2x10 6 cells) isolated from macLRP1-/- and LRP1+/+ mice were plated in 100 mm dishes in serum free media for 3 h at 37°C. Cells were scraped off the plate into 4 ml PBS and centrifuged at 1000 rpm for 5 min. Cell pellets were extracted with 300 μl … booker t washington and dubois venn diagramWebAug 19, 2010 · Levels of S100A10 on thioglycollate-elicited macrophages. Peritoneal macrophages were collected 4 days after injection with 4% thioglycollate. Cell lysates were prepared, and total levels of S100A10 or annexin A2 were examined by Western blotting (A) using actin as a loading control and quantitated (B). god of war cliffside ruins legendary chestWebFour days prior to ending of the 3-weeks therapy, all rats in three groups were injected intraperitoneally with 3% thioglycolate medium (Thermo Fisher Scientific, Waltham, MA, USA) to elicit a macrophage inflammatory response. 28 The thioglycolate-elicited peritoneal exudates (tPE) were collected as described previously at 3 weeks. 28 Jawbones ... god of war cliff of the ravenWebApr 16, 2024 · Fra-1 mRNA levels in thioglycollate-elicited macrophages peaked prominently at 1 hour, and protein levels increased 2 hours after AC or LPS challenge in vitro (Figure 2, A and B). Fra-1 expression in BMDMs following LPS stimulation also peaked 1 hour after challenge in a manner similar to that seen in thioglycollate-elicited … god of war cliffs of the ravenWebTreatment of thioglycollate-elicited, C57Bl/6 murine peritoneal macrophages with nanogram quantities of bacterial lipopolysaccharide (LPS) consistently results in altered … booker t washington and his wife