Stem loop expression
網頁2024年1月31日 · The basic helix-loop-helix (bHLH) family, one of the largest families of transcription factors in plants, is extensively involved in the growth, development, and stress response of several woody plants. However, no systematic analysis of the bHLH gene family in Quercus mongolica has been reported. We characterize QmbHLH genes and identify … 網頁2007年10月12日 · Stem-loop end-point RT-PCR analyses of miRNA expression. miRNAs were amplified using 28 cycles of PCR. Pumpkin RUBISCO ( CmRBS ) mRNA was …
Stem loop expression
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網頁HMGXB4 is a known Wnt signaling-regulating HMG-box protein, previously identified as a host-encoded factor of Sleeping Beauty (SB) transposition. Here, we show that HMGXB4 is predominantly maternally expressed, and marks both germinal progenitor and somatic stem cells. SB piggybacks HMGXB4 to activate transposase expression and target ... 莖環（英語：Stem-loop，或譯主幹-迴圈）指一種分子內鹼基配對方式，與因此形成的結構，可發生於單股DNA，但在RNA分子中較為常見。當形成的迴圈較小時，也稱為髮夾（hairpin）或髮夾環。 此種結構會在某些條件下產生，通常是因為同一分子內的兩個區域的核苷酸排列具有迴文現象，這會使兩段序列因為鹼基的配對而形成一個狀似棒棒糖的雙 …
網頁2013年1月18日 · Metazoan replication-dependent histone mRNAs have a conserved stem-loop (SL) structure at their 3′-end (1, 2), distinct from the polyadenylate tail found on all other known eukaryotic mRNAs (3, 4).The stem-loop binding protein (SLBP) (), also known as hairpin binding protein (), is a central regulator of histone mRNA metabolism. 網頁2013年1月31日 · Stem-loop RT-qPCR was developed for specific and efficient quantification of small RNAs and became a widely used technique primarily for canonical, well-characterized small RNAs [14]. An advantage of the method is that it enables the specific detection of the mature, processed miRNA molecules even from nanograms of total RNA.
網頁Approaches based on stem-loop RT In 2005, Chen et al. described the first use of a stem-loop qPCR approach for the analysis of mature miRNAs [11]. This method has been … 網頁The creation of a double His-tag fusion that forms a RNA stem loop in the mRNA encoding the N-terminus of the target protein is a novel approach for the enhancement of expression, purification, and detection of a recombinant protein. Compared to a single His-tag ...
網頁This region potentially folds into a single long stable stem-loop by pairing of intron 0 and part of exon 2. Since the coding potential for the two isoforms is identical, the interesting question arises as to the functional significance of this evolutionarily-conserved 5' …
網頁2024年3月4日 · The efficiency of stem-loop structure opening in the presence of target and non-target sequences was evaluated by fluorescence ... The aberrant expression of miR … the pixel bakery網頁2024年9月3日 · The function of the leader sequence is to fine tune expression of the trp operon based on the availability of tryptophan inside the cell. The leader sequence … side effects of psyllium網頁2024年10月28日 · By adding stop codons and stem loops 23 after a gene controlled by a constitutive promoter, we were able to reduce the basal expression of downstream … the pixel at kaset網頁The creation of a double His-tag fusion that forms a RNA stem loop in the mRNA encoding the N-terminus of the target protein is a novel approach for the enhancement of … side effects of psyllium powder網頁Inverted repeat. An inverted repeat (or IR) is a single stranded sequence of nucleotides followed downstream by its reverse complement. [1] The intervening sequence of nucleotides between the initial sequence and the reverse complement can be any length including zero. For example, 5'---TTACGnnnnnnCGTAA---3' is an inverted repeat … side effects of ptu網頁Lac Operon. a gene system whose operator gene and three structural genes control lactose metabolism in E. coli. A mutation in the O region of the lac operon would most likely result … the pixel8 rf網頁Expression studies with trpE′-′lacZtranslational fusions demonstrate that TRAP-5′ stem-loop interaction is required for proper regulation of the trp operon. 3′ RNA boundary … the pixel 4a 5g