WebbDNA-protein crosslinks (DPCs) are a specific type of DNA lesion consisting of a protein covalently and irreversibly bound to DNA, which arise after exposure to physical and chemical crosslinking agents. DPCs can be bulky and thereby pose a barrier to DNA replication and transcription. Webb31 okt. 2024 · The protocol is designed for conducting chemical cross-linking experiments of proteins and protein complexes using the MS-cleavable reagents DSBU and CDI, …
High-density chemical cross-linking for modeling protein ... - PNAS
Webb25 feb. 2024 · Methods that use a similar protocol but an ... J. L. & Ting, A. Y. Live-cell mapping of organelle-associated RNAs via proximity biotinylation combined with protein … WebbI am a University of Pennsylvania PhD and Fordham Law JD graduate with a career focus on intellectual property law and research experience in mass spectrometry, structural biology, proteomics ... chaofan rice
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WebbUV-laser-induced crosslinking produces exclusively protein-DNA adducts ( 4, 6, 8, 9) and is applicable to a broad range of protein-DNA complexes. Even complexes too weak to be seen by methods such as gel shift (electrophoretic mobility shift assay [EMSA]) or footprinting can be crosslinked. WebbThe following protocol describes crosslinking of 5 µg IgG to 50 µL Dynabeads Protein A, Dynabeads Protein G, Immunoprecipitation Kit Protein A, or Immunoprecipitation Kit Protein G.The procedure uses Pierce BS3 Crosslinker (Cat. No. 21580), which is a water-soluble crosslinker that yields irreversible (stable amide) bonds between primary amines … Webb14 dec. 2024 · Given that nuclear shuttling occurs on the msec time scale but there is a minute between treatment with peroxide and crosslinking, and that these relatively abundant proteins directly contact RNA, it seems likely that a significant fraction of the processed mRNAs found in the APEX2-NLS experiment may not be nuclear RNAs at all, … harmonized code for binders